Applications

Biotech companies must quickly convert new leads into purified therapeutics for clinical trials and ultimately for the market.  A key goal of the pilot process facility is to make GMP compliant material for testing and demonstrate a process scale-up essential to manufacturing plant design.  With an ever-increasing range of biologics (therapeutic proteins and fragment, peptides, viruses and viral capsids, naked DNA, and polysaccharides) expressed by a number of host cells (bacterial, yeast, mammalian, insect, and plant cells), the industry needs a versatile, scalable solid liquid separator to process drugs for clinical studies.  Centrifugation meets this criteria.

What is the Model APD centrifuge used for?

Celeros Model APD centrifuges have successfully been tested and approved for pilot and production scale operations in a variety of vaccine manufacturing, microbial protein, blood plasma precipitate recovery, and food additive applications.  The Celeros APD centrifuge is ideal for:

Microbial
E coli (whole cell or lysate), Inclusion Body Recovery
Lactobacillus
Streptomyces (filamentous bacteria)
Streptococcus pneumonaie
Pseudomonas flourescens

Fungal
Aspergillus (filamentous fungi)
Pichia Pastoris
Saccharomyces cerevisiae

Insect Cell
Baculovirus infected insect cell

Algae
Harvesting and washing

Mammalian
CHO (perfusion, fed batch)
MAb recovery

Blood Work
Plasma fractionation

Solid liquid separation

A variety of application notes are available today and more are being added.  If you are interested in learning about a specific process application and the performance of the Celeros APD centrifuge, simply contact Celeros and the requested documents will be provided to you by email.

Application Notes and Case Studies

  • Clarification of E. coli homogenate
  • Recovery of intracellular product derived from E. coli
  • Mammalian cell line processing
  • Harvest of bacterial derived vaccine product
  • Yield loss study to separate Lactococcus fermentation
  • Protein precipitate recovery
  • Pichia pastoris fermentation processing
  • Collagen concentration and discharge into single use bag
  • CHO cell and E. coli process shear investigation
  • Clarification of blood plasma precipitate
  • Clarification of blood plasma fractionation process
  • Inclusion body washing and concentration
  • E. coli biomass processing

Contact Celeros